Abstract
Background & Aims: The injury of the graft sustains during cold ischemia time negatively results in a poor quality of life for the patient, the need for transplanting again, and more problems in the availability of organs for transplant because liver grafts from extended criteria donors are extremely vulnerable to cold ischemia injury. Our aim is provide a new device in transplantation based on the combination of ultrasounds and hypothermia for liver transport and preservation that increases viability of liver grafts during cold ischemia before being implanted in the recipient, in comparison to what could be obtained with known devices Methods: Pig livers grafts were perfused with preservation solution at 4°C and kept in the cooler with or without ultrasound for 8 h. To assess damage induced by ischemia, transaminases and lactate dehydrogenase were measured in perfusate and caspase 3 in liver. MDA and ATP levels were determined in liver as oxidative stress and energy metabolism preservation index, respectively Results: The protection of liver grafts (confirmed by the reduction in transaminases, lactate dehydrogenase, caspase 3 and MDA as well as ATP preservation) conferred by UW solution under cold conditions (2-6oC) is higher than that obtained with Celsior or Ringer solution. A synergistic protective effect was observed because the protection obtained when both treatments (cold conditions and ultrasounds at low frequency and intensity) are combined is much better than the sum of protections obtained when both treatments are applied separately. The benefits conferred by ultrasounds were independently of the preservation solution used Conclusions: We provide a method and equipment for transporting and storing liver grafts based on hypothermal and ultrasound treatment under better conditions than those currently available. All this allows reducing the harmful effects of cold ischemia and increasing viability of grafts before they are implanted. In: Transplant International Vol. 30 (Suppl. 2), 390–576 (2017). https://doi.org/10.1121/1.5067771
BibTeX citation
@inproceedings{JimenezCastroESOT2017liver,
author = {Monica Bibiana Jiménez-Castro and Francisco Camarena and María Eugenia Cornide-Petronio and Noé Jiménez and Jordi Gracia-Sancho and Carmen Peralta},
booktitle = {ESOT 2018: 18th Congress of the European Society for Organ Transplantation. Barcelona, Spain. 24-27 September},
doi = {http://dx.doi.org/10.1111/tri.13053},
editor = {Transplant International},
isbn = {ISSN: 0934-0874},
month = {9},
number = {2},
organization = {European Society of Organ Transplantation},
pages = {390-576},
publisher = {Transplant International},
series = {Poster},
title = {A new medical device in transplantation for liver transport and preservation},
url = {http://esot2017.esot.org/},
volume = {30},
year = {2017}
}